https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49862 Wed 28 Feb 2024 15:09:16 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39583 Wed 27 Jul 2022 10:45:46 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36968 2 [IQR 7–21]) compared to benign thyroid (6 nerves/cm² [IQR: 3–10]) (p = 0.001). In contrast, no increase in nerve density was observed in FTC. In multivariate analysis, nerve density correlated positively with extrathyroidal invasion (p < 0.001), and inversely with tumour size (p < 0.001). The majority of nerves were adrenergic, although cholinergic and peptidergic innervation was detected. Perineural invasion was present in 35% of PTC, and was independently associated with extrathyroidal invasion (p = 0.008). This is the first report of infiltration of nerves into the tumour microenvironment of thyroid cancer and its association with tumour aggressiveness. The role of nerves in thyroid cancer pathogenesis should be further investigated.]]> Wed 24 May 2023 12:56:43 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47519 Wed 24 Jan 2024 14:11:41 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1527 Wed 11 Apr 2018 16:54:55 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30926 PR isoform expression ex situ and, further, to determine if conditions approaching the in vivo environment stabilise PR isoform expression in culture. Methods: Term nonlaboring human myometrial tissues were cultured under specific conditions: serum supplementation, steroids, stretch, cAMP, PMA, PGF_2α, NF-κB inhibitors, or TSA. Following 48 h culture, PR-T, PR-A, and PR-B mRNA levels were determined using qRT-PCR. PR-A/PR-B ratios were calculated. Results: PR-T and PR-A expression and the PR-A/PR-B ratio significantly increased in culture. Steroids prevented the culture-induced increase in PR-T and PR-A expression. Stretch blocked the effects of steroids on PR-T and PR-A expression. PMA further increased the PR-A/PR-B ratio, while TSA blocked culture-induced increases of PR-A expression and the PR-A/PR-B ratio. Conclusion: Human myometrial tissue in culture undergoes changes in PR gene expression consistent with transition toward a laboring phenotype. TSA maintained the nonlaboring PR isoform expression pattern. This suggests that preserving histone and/or nonhistone protein acetylation is critical for maintaining the progesterone dependent quiescent phenotype of human myometrium in culture.]]> Wed 11 Apr 2018 14:38:10 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:13858 Wed 11 Apr 2018 14:36:39 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38051 Wed 09 Aug 2023 12:59:50 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49860  rolipram > glycyl-H-1152 > HC-067047 > 2-APB > indomethacin > aminophylline. These data provide greater insight into the contraction-blocking properties of some novel tocolytics, with glycyl-H-1152, in particular, emerging as a potential novel tocolytic for preventing preterm birth.]]> Wed 07 Jun 2023 12:17:36 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34255 Ex situ analyses of human myometrial tissue has been used to investigate the regulation of uterine quiescence and transition to a contractile phenotype. Following concerns about the validity of cultured primary cells, we examined whether myometrial tissue undergoes culture-induced changes ex situ that may affect the validity of in vitro models. Objectives: To determine whether human myometrial tissue undergoes culture-induced changes ex situ in Estrogen receptor 1 (ESR1), Prostaglandin-endoperoxide synthase 2 (PTGS2) and Oxytocin receptor (OXTR) expression. Additionally, to determine whether culture conditions approaching the in vivo environment influence the expression of these key genes. Methods: Term non-laboring human myometrial tissues were cultured in the presence of specific treatments, including; serum supplementation, progesterone and estrogen, cAMP, PMA, stretch or NF-κB inhibitors. ESR1, PTGS2 and OXTR mRNA abundance after 48 h culture was determined using quantitative RT-PCR. Results: Myometrial tissue in culture exhibited culture-induced up-regulation of ESR1 and PTGS2 and down-regulation of OXTR mRNA expression. Progesterone prevented culture-induced increase in ESR1 expression. Estrogen further up-regulated PTGS2 expression. Stretch had no direct effect, but blocked the effects of progesterone and estrogen on ESR1 and PTGS2 expression. cAMP had no effect whereas PMA further up-regulated PTGS2 expression and prevented decline of OXTR expression. Conclusion: Human myometrial tissue in culture undergoes culture-induced gene expression changes consistent with transition toward a laboring phenotype. Changes in ESR1, PTGS2 and OXTR expression could not be controlled simultaneously. Until optimal culture conditions are determined, results of in vitro experiments with myometrial tissues should be interpreted with caution.]]> Wed 04 Sep 2019 10:05:05 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47796 Tue 31 Jan 2023 15:32:49 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44767 Tue 30 May 2023 11:48:47 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36640 Tue 25 Oct 2022 09:27:51 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23393 Tue 24 Apr 2018 11:33:54 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54561 Thu 29 Feb 2024 12:33:44 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32097 Thu 28 Oct 2021 12:37:05 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51190 Thu 24 Aug 2023 14:39:18 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41024 Thu 21 Jul 2022 12:15:19 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32094 Thu 17 Mar 2022 14:40:51 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37077 Thu 13 Aug 2020 16:17:43 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38752 Mon 24 Jan 2022 10:28:08 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38750 Mon 24 Jan 2022 10:21:02 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32092 Mon 23 Sep 2019 12:35:23 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47510 Mon 23 Jan 2023 12:08:39 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50905 Mon 13 Nov 2023 08:50:41 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45462 Fri 28 Oct 2022 14:41:08 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36624 Fri 21 Oct 2022 12:10:07 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39927 Fri 15 Jul 2022 10:34:45 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32098 Fri 03 Dec 2021 10:35:09 AEDT ]]>